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Antibodies bound to the blocking peptide no more bind for the epitope on the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is unique.
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This guideline outlines additional doable optimization of mobile and immunoassay parameters to ensure the absolute best results are...
This information outlines further more achievable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide typically has the epitope identified because of the antibody.
Target Info Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress as a result of S stage.
Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and therefore could lead to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial function in marketing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants check here of the gene, which encode unique isoforms, have already been explained. Two additional splice variants have been claimed but in depth nucleotide sequence information and facts just isn't still accessible.
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